Novel Liquid Biopsy – Chromatin immunoprecipitation (ChIP) of Circulating CTCF Transcription Factor-DNA Nucleoproteins from Plasma Isolates Pure Tumor Derived ctDNA Sequences for Analysis by PCR
Time: 1:20 pm
day: Day Two Track A AM
Details:
- Tumor derived ctDNA relating to cancer associated gain of occupancy CTCF binding sites circulates as CTCF-DNA nucleoproteins in cancer patients but as nucleosomes in all other patients
- Pure ctDNA gain of occupancy CTCF binding site sequences are isolated by CTCF ChIP with removal of all non-tumor derived nucleosome covered cfDNA fragments
- Liquid biopsy of the isolated pure ctDNA sequence by PCR is an accurate, low cost, rapid, automatable method for detection of early stage solid and liquid cancers
